Atomistic Insights into the Unique Roles of Lipopolysaccharide Modifications in Strengthening Bacterial Outer Membrane Defenses
نویسندگان
چکیده
منابع مشابه
Lipopolysaccharide transport to the bacterial outer membrane in spheroplasts.
The mechanism of lipopolysaccharide (LPS) transport in Gram-negative bacteria from the inner membrane to the outer membrane is largely unknown. Here, we investigated the possibility that LPS transport proceeds via a soluble intermediate associated with a periplasmic chaperone analogous to the Lol-dependent transport mechanism of lipoproteins. Whereas newly synthesized lipoproteins could be rele...
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Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis. However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells. Here, we describe a highly s...
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Although previous studies have demonstrated that lipopolysaccharide (LPS) and the outer membrane protein OmpC are required for bacteriophage T4 infectivity in Escherichia coli, it is unclear whether they played a role in binding or in the downstream viral processes that followed. Such processes may vary from phage DNA injection to viral gene expression, assembly, or release. To investigate this...
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This review presents a brief outline of our current knowledge of the structure and chemical composition of the outer membrane vesicles (OMVs), originating from the surface of Gram negative bacteria including their outer membrane proteins and lipopolysaccharides. Moreover, the functional roles and applications of OMVs in medical research such as OMV-based vaccines, OMV adjuvants properties, OMV ...
متن کاملLipopolysaccharide transport to the cell surface: periplasmic transport and assembly into the outer membrane.
Gram-negative bacteria possess an outer membrane (OM) containing lipopolysaccharide (LPS). Proper assembly of the OM not only prevents certain antibiotics from entering the cell, but also allows others to be pumped out. To assemble this barrier, the seven-protein lipopolysaccharide transport (Lpt) system extracts LPS from the outer leaflet of the inner membrane (IM), transports it across the pe...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2018
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2017.11.1556